Actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. It has not previously been reported. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and. Proc Natl Acad Sci U S A Sep 29; Draft Genome Sequence of Actinomadura madurae LIID-AJ, Isolated from a Human Mycetoma Case. Vera-Cabrera L.

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Tracking a hospital outbreak of carbapenem-resistant Klebsiella pneumoniae with whole-genome sequencing. Address correspondence to Stephen J. Deep sequencing has previously been used to characterize cultured bacterial isolates 27 and to explore the composition of microbial populations in metagenomic research 28 A systematic approach for discovering novel, clinically relevant bacteria.

Info – Actinomadura madurae DSM

In other projects Wikimedia Commons Wikispecies. Actinomadura madurae is an aerobic actinomycete which is best known worldwide catinomadura the cause of actinomycotic mycetomas. Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial actionmadura. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms.

Intravenous penicillin 20 million units i.

Molecular Diagnosis of Actinomadura madurae Infection by 16S rRNA Deep Sequencing

The portion of the biopsy material sequenced may have actinomadjra little of the aerobic actinomycetes inclusion, which represented a focal area within the larger sample Fig. A two-step schedule for the treatment of actinomycotic mycetomas. QIDciprofloxacin mg p. A modified two-step treatment for actinomycetoma.

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Departments of Laboratory Medicine a. Treatment of actinomycetoma foot—our experience with ten patients. Actinomadura Slide culture of Actinobacteria Scientific classification Kingdom: Abstract Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial actinomadrua. This article has been cited by other articles in PMC. Chronic infections 7 and diabetic foot ulcers 589in particular, can contain highly diverse bacterial populations.


By using this site, you agree to the Terms of Use and Privacy Policy. A punch biopsy specimen of the lesion was submitted to pathology, revealing marked acute and chronic inflammation and an inclusion of abundant filamentous structures consistent with aerobic actinomycetes Fig.

Actinomadura Lechevalier and Lechevalier Journal List J Clin Microbiol v. Although the patient remained ambulatory throughout the course of her disease, she gradually had to modify her footwear to accommodate the increasing size of her foot, which at the time of presentation was causing her great pain. For some sequences, multiple organisms met the identity threshold for species-level classification; in such cases, species names separated by a slash and genus names separated by a semicolon list the possible taxonomic assignments.

Footnotes Published ahead of print 9 October Thus, the relative abundance of A. Open in a separate window. Metagenomic systems biology of the human gut microbiome reveals topological shifts associated with obesity and inflammatory bowel disease.

Table 1 details the classification of deep-sequencing reads from the specimen submitted for analysis. Consistent with culture results, reads classified as S. The isolate from the patient’s blood was subjected to dilutional antimicrobial susceptibility tests with 12 antimicrobial agents and was found to have a wide spectrum of susceptibility. A portion of the specimen was concurrently submitted for culture in order to establish the identity of the aerobic actinomycetes-like organism and to evaluate its antibiotic sensitivities; however, the culture quickly became overgrown by Staphylococcus aureuspreventing culture for slower-growing aerobic actinomycetes madrae.

Given the extent of infection, neither additional antibiotic therapy nor debridement with subsequent reconstruction was felt to be a viable option, and a recommendation was made for below-the-knee amputation.

National Center for Biotechnology InformationU. Further, it should be noted that read counts in 16S rRNA amplicon deep-sequencing studies are semiquantitative and correlate only roughly with the relative abundances of organisms due to bias introduced through PCR 23maduuraediffering DNA extraction efficiencies for particular organisms 25and organism-specific differences in 16S rRNA operon counts A number of other organisms were also detected, reflecting the polymicrobial nature of such specimens 5.


Cummingsa Bronwyn H. Table 1 Deep-sequencing results. Hoogestraatmadurar Lisa A.

Comparison of DNA extraction methods for microbial community profiling with an application to pediatric bronchoalveolar lavage samples. Based on these findings, the diagnosis of actinomycotic mycetoma was suggested. Actinomycetoma in the Maduraee States. The consensus sequence determined in this work has been submitted to GenBank under accession no.

From Wikipedia, the free encyclopedia. In this case report, we have utilized the approach to investigate the causative agent of biopsy-proven actinomycotic mycetoma.

Nonmycetomic Actinomadura madurae infection in a patient with AIDS.

Hamilton Bailey’s demonstrations of physical signs in clinical surgery18th ed. Int J Syst Evol Microbiol. Both of these diagnostic limitations complicated analysis of the patient specimen in this report, a biopsy specimen from a diabetic foot ulcer.

Published ahead of print 9 October This Actinobacteria -related article is a stub. In one small, prospective study, initial treatment with intravenous gentamicin, intravenous penicillin, and oral sulfamethoxazole-trimethoprim followed by oral sulfamethoxazole-trimethoprim and oral amoxicillin maintenance was found effective in empirically treating disease minimally involving the bones, while actinomaudra incorporating intravenous amikacin and oral sulfamethoxazole-trimethoprim for initial treatment and oral sulfamethoxazole-trimethoprim for maintenance therapy were useful in cases of more extensive bony involvement